Q: Is the ADN432 New compatible with my LightCycler 480? A: Yes. The excitation at 498nm is within the 470-505nm range of the LightCycler’s “483” channel.
Q: Does the "New" formula inhibit reverse transcription? A: No. In fact, the ADN432 New has been validated in One-Step RT-qPCR using M-MLV and Superscript IV. It shows less inhibition than SYBR Green at equivalent volumes.
Q: Can I use ADN432 New for digital PCR (dPCR)? A: Yes, specifically the lyophilized bead version. The improved thermal stability prevents droplet evaporation during the partitioning step. adn432 new
Q: Where can I find the certificate of analysis (COA) for lot-specific ADN432 New? A: Visit the manufacturer’s portal and enter the lot number found on the box. The ADN432 New batches all include a melt curve validation graph generated on a QuantStudio 7 Flex.
For the first time in the ADN432 line, the ADN432 New is available in a lyophilized bead format. This is ideal for field diagnostics and point-of-care (POC) applications where cold chain logistics are unreliable. Q: Is the ADN432 New compatible with my LightCycler 480
Q: Is ADN432 new compatible with in vivo work?
A: Yes. Multiple labs have reported successful intravenous and intracerebroventricular administration in mice at doses up to 25 mg/kg without acute toxicity. Always run pilot tolerability studies for your specific model.
Q: Does the “new” version cross the blood-brain barrier?
A: Unmodified ADN432 new does not cross the BBB efficiently. However, the manufacturer offers a lipid-nanoparticle (LNP) encapsulated version (ADN432 new-LNP) that increases brain delivery to ~4% of injected dose. Q: Does the "New" formula inhibit reverse transcription
Q: Can I use ADN432 new with standard RT-qPCR reagents?
A: Yes, but note that its high nuclease resistance means it will not degrade during cDNA synthesis. Ensure your reverse transcriptase is heat-inactivated (85°C for 5 min) to avoid interference in downstream amplification.