| Metric | Sequencher 414 | MinION Mk1C | Flongle | |--------|----------------|-------------|---------| | Active pores | 414 | 512 (but typical 200–400) | 126 | | Throughput (72h) | 25 Gb | 15 Gb | 2.5 Gb | | Power (W) | 3.5 (peak) | 12 | 2.5 (USB) | | Weight (g) | 180 | 350 (with screen) | 15 (dongle) | | On-device basecalling | Yes (ASIC) | Yes (CPU/GPU) | No (host required) | | Adaptive sampling | Per-pore RL | Bulk voltage reversal | No |
Read length N50: 15 kb (can exceed 100 kb with high-molecular-weight DNA).
You have two options:
The 414’s high sampling rate (40 kHz) allows detection of 5mC and 6mA via residual current deviations, with sensitivity of 92% and specificity 94% compared to bisulfite sequencing.
How does this specific 414 configuration stack up against other portable sequencers? portable sequencher 414
| Feature | Portable Sequencher 414 | Illumina iSeq 100 | Oxford Nanopore MinION Mk1C | | :--- | :--- | :--- | :--- | | Weight | 8.5 kg (18.7 lbs) | 45 kg (99 lbs) | 1.5 kg (3.3 lbs) | | Read Length | >100 kb | 2x150 bp (short) | >100 kb | | Time to Result | 4-6 hours | 24 hours | 6-12 hours | | Onboard Compute | Yes (RTX 4060) | No (requires laptop) | Yes (limited CPU only) | | Power Requirement | 140W DC | 800W AC | 45W DC | | Best For | Full genome assembly in field | Targeted panels | Rapid pathogen ID |
The 414 rig’s advantage is the balance: it has more compute power than the Mk1C (which uses an old NVIDIA Jetson TX2) and is far more portable than the iSeq. | Metric | Sequencher 414 | MinION Mk1C
A piece of hardware is useless without the intelligence to interpret squiggly electrical signals. The "Sequencher" part of the name is critical. Traditionally, Sequencher by Gene Codes is used for Sanger and NGS data. However, the Portable 414 variant utilizes a streamlined, command-line–friendly version of this software or its open-source analogues.